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Expression cassette

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An expression cassette is a distinct component of vector DNA consisting of a gene and regulatory sequence to be expressed by a transfected cell. In each successful transformation, the expression cassette directs the cell's machinery to make RNA and protein(s). Some expression cassettes are designed for modular cloning of protein-encoding sequences so that the same cassette can easily be altered to make different proteins.

An expression cassette is composed of one or more genes and the sequences controlling their expression. An expression cassette comprises three components: a promoter sequence, an open reading frame, and a 3' untranslated region that, in eukaryotes, usually contains a polyadenylation site.

Different expression cassettes can be transfected into different organisms including bacteria, yeast, plants, and mammalian cells as long as the correct regulatory sequences are used.

See also

References

  1. Papadakis, E.D.; et al. (2004). "Promoters and Control Elements: Designing Expression Cassettes for Gene Therapy". Current Gene Therapy. 4 (4): 89–113. CiteSeerX 10.1.1.494.3472. doi:10.2174/1566523044578077. PMID 15032617.
  2. Bertrand, Edouard; et al. (1997). "The expression cassette determines the functional activity of ribozymes in mammalian cells by controlling their intracellular localization". RNA. 3 (1): 75–88. PMC 1369464. PMID 8990401.
  3. Vickers, Claudia E (December 2013). "Dual gene expression cassette vectors with antibiotic selection markers for engineering in Saccharomyces cerevisiae". Microbial Cell Factories. 12 (96): 96. doi:10.1186/1475-2859-12-96. PMC 4231455. PMID 24161108.
  4. Gray, Steven James; et al. (January 2015). "Viral Expression Cassette Elements to Enhance Transgene Target Specificity and Expression in Gene Therapy". Discov Med. 19 (102): 49–57. ISSN 1539-6509. PMC 4505817. PMID 25636961.
  5. Azhakanandam, K.; et al. (2015). "Plant Trait Gene Expression Cassette Design". Recent Advancements in Gene Expression and Enabling Technologies in Crop Plants. Springer. pp. 41–52. doi:10.1007/978-1-4939-2202-4_2. ISBN 978-1-4939-2201-7. S2CID 82188623.
  6. Chow, Yu-Hua; et al. (December 1997). "Development of an epithelium-specific expression cassette with human DNA regulatory elements for transgene expression in lung airways" (PDF). Proceedings of the National Academy of Sciences. 94 (26): 14695–14700. Bibcode:1997PNAS...9414695C. doi:10.1073/pnas.94.26.14695. PMC 25096. PMID 9405675.
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