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NS0 cell

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NS0 cells are a model cell line derived from the nonsecreting murine myeloma used in biomedical research and commercially in the production of therapeutic proteins. The cell line is a cholesterol-dependent cell line that was generated from a subline of NSI/1 which produced only the light chain but no heavy chain.

Cell line development

Development of murine neoplasms started with work with the BALB/c mice to isolate the IgG1 secreting MOPC21 tumor. From this tumor, the P3K cells were isolated and developed into two cell lines, 289-16 and P3-X63. The 289-16 cell line secreted only light chain and no heavy chain and was renamed NSI/1. Clones from that cell line were isolated and a nonsecreting cell line was identified and was named NS0/1.

Uses in biotechnology

As myeloma cells, NSo cells are naturally antibody-producing suspension cells with a lymphoblast morphology. Gene amplification is typically performed using GS-transfected NS0 cells to select for producing cell lines. The GS-NS0 is a heterologous mammalian expression system that allows for the rapid expression of recombinant proteins. Several therapeutic antibody products are produced using the NS0 cell line including daclizumab and eculizumab.

References

  1. Barnes, LM; Bentley, CM; Dickson, AJ (February 2000). "Advances in animal cell recombinant protein production: GS-NS0 expression system". Cytotechnology. 32 (2): 109–23. doi:10.1023/A:1008170710003. PMC 3449689. PMID 19002973.
  2. ^ M. Barnes, Louise; Bentley, Catherine M.; Dickson, Alan J. (2000). "Advances in animal cell recombinant protein production: GS-NS0 expression system". Cytotechnology. 32 (2): 109–123. doi:10.1023/A:1008170710003. PMC 3449689. PMID 19002973.
  3. Sato, JD; Cao, HT; Kayada, Y; Cabot, MC; Sato, GH; Okamoto, T; Welsh, CJ (December 1988). "Effects of proximate cholesterol precursors and steroid hormones on mouse myeloma growth in serum-free medium". In Vitro Cellular & Developmental Biology. 24 (12): 1223–8. doi:10.1007/bf02624194. PMID 3209588. S2CID 2470410.
  4. Galfrè, G; Milstein, C (1981). "[1] Preparation of monoclonal antibodies: Strategies and procedures". Preparation of monoclonal antibodies: strategies and procedures. Methods in Enzymology. Vol. 73. pp. 3–46. doi:10.1016/0076-6879(81)73054-4. ISBN 9780121819736. PMID 7300683.
  5. "NS0 Cell Line from murine myeloma". Retrieved 15 July 2018.
  6. Barnes, Louise M.; Bentley, Catherine M.; Dickson, Alan J. (20 May 2001). "Characterization of the stability of recombinant protein production in the GS-NS0 expression system". Biotechnology and Bioengineering. 73 (4): 261–270. doi:10.1002/bit.1059. PMID 11283909.
  7. Wurm, Florian M (November 2004). "Production of recombinant protein therapeutics in cultivated mammalian cells". Nature Biotechnology. 22 (11): 1393–8. doi:10.1038/nbt1026. PMID 15529164. S2CID 20428452.

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