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Otoconin

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Protein family
OC90
Identifiers
AliasesOC90, PLA2L, otoconin 90
External IDsOMIM: 601658; MGI: 1313269; HomoloGene: 19021; GeneCards: OC90; OMA:OC90 - orthologs
Gene location (Human)
Chromosome 8 (human)
Chr.Chromosome 8 (human)
Chromosome 8 (human)Genomic location for OC90Genomic location for OC90
Band8q24.22Start132,024,216 bp
End132,059,382 bp
Gene location (Mouse)
Chromosome 15 (mouse)
Chr.Chromosome 15 (mouse)
Chromosome 15 (mouse)Genomic location for OC90Genomic location for OC90
Band15 D1|15 29.16 cMStart65,747,902 bp
End65,784,246 bp
RNA expression pattern
Bgee
HumanMouse (ortholog)
Top expressed in
  • testicle

  • substantia nigra

  • placenta

  • prefrontal cortex

  • Hypothalamus

  • Brodmann area 9

  • anterior cingulate cortex

  • subcutaneous adipose tissue

  • Pituitary Gland
Top expressed in
  • vestibular sensory epithelium

  • saccule

  • otic vesicle

  • utricle

  • entorhinal cortex

  • perirhinal cortex

  • outer nuclear layer

  • morula

  • CA3 field

  • embryo
More reference expression data
BioGPS
n/a
Gene ontology
Molecular function
Cellular component
Biological process
Sources:Amigo / QuickGO
Orthologs
SpeciesHumanMouse
Entrez

729330

18256

Ensembl

ENSG00000253117

ENSMUSG00000015001

UniProt

Q02509

Q9Z0L3

RefSeq (mRNA)

NM_001080399

NM_010953
NM_001347038

RefSeq (protein)

NP_001073868

NP_001333967
NP_035083

Location (UCSC)Chr 8: 132.02 – 132.06 MbChr 15: 65.75 – 65.78 Mb
PubMed search
Wikidata
View/Edit HumanView/Edit Mouse
Oc90
Identifiers
AliasesOcn-95PLA2LPla2lloc95otoconin 90
External IDsHomoloGene: 19021; GeneCards: ; OMA:- orthologs
Gene location (Human)
Chromosome 15 (human)
Chr.Chromosome 15 (human)
Chromosome 15 (human)Genomic location for Oc90Genomic location for Oc90
Band15 D1|15 29.16 cMStart65,747,902 bp
End65,784,246 bp
RNA expression pattern
Bgee
HumanMouse (ortholog)
Top expressed in
  • vestibular sensory epithelium

  • saccule

  • otic vesicle

  • utricle

  • entorhinal cortex

  • perirhinal cortex

  • outer nuclear layer

  • morula

  • CA3 field

  • embryo
    n/a
More reference expression data
BioGPS
n/a
Gene ontology
Molecular function
Cellular component
Biological process
Sources:Amigo / QuickGO
Orthologs
SpeciesHumanMouse
Entrez

18256

n/a

Ensembl

ENSMUSG00000015001

n/a

UniProt

Q9Z0L3

n/a

RefSeq (mRNA)

NM_010953
NM_001347038

n/a

RefSeq (protein)

NP_001333967
NP_035083

n/a

Location (UCSC)Chr 15: 65.75 – 65.78 Mbn/a
PubMed searchn/a
Wikidata
View/Edit Human

Otoconin is a structural glycoprotein found in the otoconial membrane of vertebrates. It is the major protein component of the otoconia (ear dust).

In mice the protein is called otoconin-90, and is coded by the Ocn-95 gene. In humans the ortholog is called PLA2L, originally believed to be a pseudogene.

In mice the protein contains 469 amino acids, and is coded by 1906 base-pair DNA. In mice the protein is first formed at day 9.5 in the otic vesicle dorsal wall epithelium, and also in the endolymphatic duct. This is before any minerals are deposited. Four days later it also appears in the non-sensory epithelium of the utricle and saccule and semicircular canals. The roof of the cochlear duct also has some. By ten days after birth the protein is not found in any cells, but only in the acellular membranes.

The otoconin-90 contains two secretory phospholipase A2 (sPLA2) domains. sPLA2 has a rigid structure with eight disulfide bonds. The sPLA2 domain has the ability to bind a phospholipid, but does not have enzymatic capability. Presumably the domain also binds calcium Ca ions and carbonate CO3. Otoconin-90 would position the ions to fit into a calcite lattice. The calcium is hypothesized to be secreted from the sensory epithelium into the gelatinous substance on the top of which it meets otoconin and forms otoconia.

In amphibians (Xenopus) a similar mass protein is contained in the utricle along with calcite. The saccule contains aragonite with otoconin-22 which is 22 kDa in mass. Otoconin-22 contains 127 amino acids. Otoconin-22 has a single sPLA2 domain.

Chondrostean fish contain a similar protein of 40 to 50 kDa mass, and vaterite as the mineral component.

In mammals otoconin makes up 90% of the protein component of an otoconium grain, primarily in the core.

References

  1. ^ GRCh38: Ensembl release 89: ENSG00000253117Ensembl, May 2017
  2. ^ GRCm38: Ensembl release 89: ENSMUSG00000015001Ensembl, May 2017
  3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  6. ^ Verpy, E.; Leibovici, M.; Petit, C. (19 January 1999). "Characterization of otoconin-95, the major protein of murine otoconia, provides insights into the formation of these inner ear biominerals". Proceedings of the National Academy of Sciences. 96 (2): 529–534. Bibcode:1999PNAS...96..529V. doi:10.1073/pnas.96.2.529. PMC 15170. PMID 9892667.
  7. ^ Thalmann, Ruediger; Ignatova, Elena; Kachar, Bechara; Ornitz, David M.; Thalmann, ISOLDE (25 January 2006). "Development and Maintenance of Otoconia". Annals of the New York Academy of Sciences. 942 (1): 162–178. doi:10.1111/j.1749-6632.2001.tb03743.x. PMID 11710459. S2CID 85626725.
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