Protoculture may refer to:
- Protoculture, the passing of learned behaviors from one generation to another in non-human primates
- A term used in the science fiction anime series The Super Dimension Fortress Macross, but referring to different things depending on the version:
- In Macross: an ancient race of alien humanoids
- In the American adaptation, Robotech: an ancient power source and foodstuff
- Protoculture Addicts, a North American anime and manga magazine
- Protoculture Inc., publisher of the magazine
- "Proto Culture", a hip-hop song about video games, by artist Del tha Funkee Homosapien (featuring Khaos Unique), on the album Both Sides of the Brain
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