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RMND5B

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Protein-coding gene in the species Homo sapiens
RMND5B
Identifiers
AliasesRMND5B, GID2, GID2B, required for meiotic nuclear division 5 homolog B
External IDsMGI: 1913339; HomoloGene: 100777; GeneCards: RMND5B; OMA:RMND5B - orthologs
Gene location (Human)
Chromosome 5 (human)
Chr.Chromosome 5 (human)
Chromosome 5 (human)Genomic location for RMND5BGenomic location for RMND5B
Band5q35.3Start178,130,996 bp
End178,150,568 bp
Gene location (Mouse)
Chromosome 11 (mouse)
Chr.Chromosome 11 (mouse)
Chromosome 11 (mouse)Genomic location for RMND5BGenomic location for RMND5B
Band11|11 B1.3Start51,514,498 bp
End51,526,723 bp
RNA expression pattern
Bgee
HumanMouse (ortholog)
Top expressed in
  • left testis

  • right testis

  • apex of heart

  • gastrocnemius muscle

  • muscle of thigh

  • prefrontal cortex

  • epithelium of esophagus

  • skin of leg

  • anterior pituitary

  • ectocervix
Top expressed in
  • granulocyte

  • interventricular septum

  • spermatid

  • hair follicle

  • lip

  • spermatocyte

  • morula

  • dentate gyrus of hippocampal formation granule cell

  • seminiferous tubule

  • right kidney
More reference expression data
BioGPS


More reference expression data
Gene ontology
Molecular function
Cellular component
Biological process
Sources:Amigo / QuickGO
Orthologs
SpeciesHumanMouse
Entrez

64777

66089

Ensembl

ENSG00000145916

ENSMUSG00000001054

UniProt

Q96G75

Q91YQ7

RefSeq (mRNA)

NM_001288794
NM_001288795
NM_022762

NM_025346

RefSeq (protein)

NP_001275723
NP_001275724
NP_073599

NP_079622

Location (UCSC)Chr 5: 178.13 – 178.15 MbChr 11: 51.51 – 51.53 Mb
PubMed search
Wikidata
View/Edit HumanView/Edit Mouse

Required for meiotic nuclear division 5 homolog B (S. cerevisiae), also known as RMND5B, is a protein which in humans is encoded by the RMND5B gene. It has a zinc finger domain and is highly conserved throughout many eukaryotic organisms.

Protein sequence

This protein is rich in leucine (14.0%) and might belong to the protein family of leucine-rich repeats 

        1 meqcacvere ldkvlqkflt ygqhcersle ellhyvgqlr aelasaalqg tplsatlslv
       61 msqccrkikd tvqklasdhk dihssvsrvg kaidrnfdse icgvvsdavw dareqqqqil
      121 qmaivehlyq qgmlsvaeel cqestlnvdl dfkqpfleln rilealheqd lgpalewavs
      181 hrqrllelns slefklhrlh firllaggpa kqlealsyar hfqpfarlhq reiqvmmgsl
      241 vylrlgleks pychlldssh waeicetftr dacsllglsv esplsvsfas gcvalpvlmn
      301 ikavieqrqc tgvwnhkdel pieielgmkc wyhsvfacpi lrqqtsdsnp piklicghvi
      361 srdalnklin ggklkcpycp meqnpadgkr iif

Homology

text shaded multiple sequence alignment of CAD28476; the shaded amino acids are conserved

CAD28476 is highly conserved in many eukaryotic organism. Its high conservation suggests that it plays a primary role in meiosis.

Orthologs

taxonomic name common name NCBI entry Percentage of sequence similarity Length (AAs) comments
Homo sapiens Human 100% 393 hypothetical Protein for Meiosis
Pan troglodytes Chimpanzee 99.7% 393 required for meiotic nuclear division 5 homolog B isoform 6
Macaca mulatta Rhesus macaque 98.5% 393 Similar to CG3295-PA isoform 11
Rattus norvegicus Norway rat 98.2% 393 Similar to RIKEN cDNA 0610039K22
Mus musculus House mouse 97.7% 393 Required for meiotic nuclear division 5 homolog B
Equus caballus Horse 97.7% 273 PREDICTED: similar required for meiotic nuclear division 5 homolog B
Bos taurus Cattle 95.4% 393 required for meiotic nuclear division 5 homolog B
Danio rerio Zebrafish 72.8% 391 required for meiotic nuclear division 5 homolog B
Xenopus laevis African clawed frog 70% 391 MGC84431 protein
Canis lupus familiaris Dog 70% 391 PREDICTED: similar to CG3295-PA isoform A
Tetraodon nigroviridis Spotted green pufferfish 68.5% 417 unnamed protein product
Ornithorhynchus anatinus Platypus 64.5% 389
Branchiostoma floridae Florida lancelet 57% 491 hypothetical protein BRAFLDRAFT_126901
Nematostella vectensis Sea anemone 50.1% 389
Culex quinquefasciatus Southern house mosquito 48.5% 392 conserved hypothetical protein
Aedes aegypti Yellow fever mosquito 48.2% 392 hypothetical protein AaeL_AAEL009407
Strongylocentrotus purpuratus Purple urchin 48.2 405 PREDICTED: Similar to MGC88921 protein
Drosophila virilis Fruit fly 41.5% 437 GJ20343
Drosophila melanogaster Fruit fly 40.3% 431 CG3295
Acyrthosiphon pisum Acrythosiphon 39.5% 366 PREDICTED: required for meiothic nuclear division 5 homolg A
Oryza sativa Rice 32.2% 386 membrane protein-like

Zinc finger domain

This depiction shows the location of the two predicted protein domains.
Result of local sequence alignment- the amino acids found in the zinc finger domain are highlighted in different color
The three amino acids which are coordinated to the zinc ion.

Two domains were predicted by the program BLIMPS to exist in the protein of which one of the domains contains a zinc finger domain.

Zinc finger domains assist the binding of the protein to nucleic acids. This points to a direct interaction of CAD28476 with DNA during meiosis. By comparing CAD28476 with a related zinc finger protein in a local sequence alignment using LALIGN, the amino acids His359, Cys381 and Cys384 could be attributed to the zinc finger domain. This zinc finger structure is uncommon in the way that it involves one histidine instead of two.

Expression

Microarray data show that CAD28476 is highly expressed in tissue where meiosis occurs like in testis and ovaries. Moreover, it is also highly expressed in the brain around the hypothalamus.

Transcriptional regulation

Listed binding sites for different transcription factors, the bold transcription factors are very likely to be involved in regulation.

The analysis of the promoter region (tools on the page rVista . were used) shows that there are several transcription factor binding sites localized in conserved regions . It is very likely that the transcription factor Ets-1 which belongs to the ETS transcription factor family and its core binding factor CBF are involved in regulation of transcription since they both have independent binding sites.

Interacting proteins

There were two proteins predicted which interact with CAD28476.

name of the protein Uniprot entry information about the gene
Ewing sarcoma breakpoint region 1, isoform CRA_a gene encodes a putative RNA binding protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors.
Mothers against decapentaplegic homolog 4 SMAD4

References

  1. ^ GRCh38: Ensembl release 89: ENSG00000145916 – Ensembl, May 2017
  2. ^ GRCm38: Ensembl release 89: ENSMUSG00000001054 – Ensembl, May 2017
  3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. "Entrez Gene: RMND5B required for meiotic nuclear division 5 homolog B (S. cerevisiae)".
  6. Jorja Henikoff, Fred Hutchinson Cancer Research Center, 1100 Fairview AV N, A1-162, PO Box 19024 Seattle, WA 98109-1024 FAX: 206-667-5889
  7. Miyamoto, K.; Tochio, N.; Sato, M.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S. (2005). "PDB entry of 2ct2". Worldwide Protein Data Bank. doi:10.2210/pdb2ct2/pdb. Retrieved 12 May 2009.
  8. © 1997 by William R. Pearson and the University of Virginia (This is from distribution "fasta20u66", version 2.0u66, Sep., 1998, sale or incorporation into a commercial product expressly forbidden without permission)
  9. "rvista Home Page". Retrieved 12 May 2009.
  10. "homepage of Genecard". Retrieved 12 May 2009.

Further reading

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