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| {{mergeto|Agranulocyte|discuss=Talk:Agranulocyte|date=February 2010}} | {{mergeto|Agranulocyte|discuss=Talk:Agranulocyte|date=February 2010}} | ||
| A '''peripheral blood mononuclear cell''' ('''PBMC''') is any ] ] having a round nucleus<ref>Delves, Peter, et al. Roitt's Essential Immunology, 11th Ed. ISBN |
A '''peripheral blood mononuclear cell''' ('''PBMC''') is any ] ] having a round nucleus<ref>Delves, Peter, et al. Roitt's Essential Immunology, 11th Ed. ISBN 978-1-4051-3603-7</ref>. For example: a ], a ] or a ]. These blood cells are a critical component in the ] to fight infection and adapt to intruders. The lymphocyte population consists of T cells (CD4 and CD8 positive ~75%), B cells and NK cells (~25% combined). | ||
| These cells are often extracted from whole blood using ], a hydrophilic polysaccharide that separates layers of blood, with ] and ] forming a ] under a layer of plasma. This buffy coat contains the PBMCs. Additionally, PBMC can be extracted from whole blood using a hypotonic lysis which will preferentially lyse red blood cells. This method results in neutrophils and other polymorphonuclear (PMN) cells which are important in innate immune defence being obtained. | These cells are often extracted from whole blood using ], a hydrophilic polysaccharide that separates layers of blood, with ] and ] forming a ] under a layer of plasma. This buffy coat contains the PBMCs. Additionally, PBMC can be extracted from whole blood using a hypotonic lysis which will preferentially lyse red blood cells. This method results in neutrophils and other polymorphonuclear (PMN) cells which are important in innate immune defence being obtained. | ||
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| == Research Applications == | == Research Applications == | ||
| Many scientists conducting research in the fields of ] (including ] disorders), ], ], ] development, ], and ] are frequent users of '''Peripheral Blood Mononuclear Cells'''. In many cases, PBMCs are derived from ]. | Many scientists conducting research in the fields of ] (including ] disorders), ], ], ] development, ], and ] are frequent users of '''Peripheral Blood Mononuclear Cells'''. In many cases, PBMCs are derived from ]. | ||
| ⚫ | ==References== | ||
| ⚫ | <references/> | ||
| {{DEFAULTSORT:Pbmc}} | {{DEFAULTSORT:Pbmc}} | ||
| ] | ] | ||
| ⚫ | ==References== | ||
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| {{Cell-biology-stub}} | {{Cell-biology-stub}} | ||
Revision as of 17:46, 9 September 2010
For the internet slang term "PBMC", see ProblemBetweenMonitorChair. | It has been suggested that this article be merged into Agranulocyte. (Discuss) Proposed since February 2010. |
A peripheral blood mononuclear cell (PBMC) is any blood cell having a round nucleus. For example: a lymphocyte, a monocyte or a macrophage. These blood cells are a critical component in the immune system to fight infection and adapt to intruders. The lymphocyte population consists of T cells (CD4 and CD8 positive ~75%), B cells and NK cells (~25% combined).
These cells are often extracted from whole blood using ficoll, a hydrophilic polysaccharide that separates layers of blood, with monocytes and lymphocytes forming a buffy coat under a layer of plasma. This buffy coat contains the PBMCs. Additionally, PBMC can be extracted from whole blood using a hypotonic lysis which will preferentially lyse red blood cells. This method results in neutrophils and other polymorphonuclear (PMN) cells which are important in innate immune defence being obtained.
PBMCs are widely used in research and clinical uses every day. HIV research uses them because PBMCs include CD4+ cells, which are the cells HIV infects.
See also Peripheral blood cell.
Research Applications
Many scientists conducting research in the fields of immunology (including auto-immune disorders), infectious disease, hematological malignancies, vaccine development, Transplant Immunology, and high-throughput screening are frequent users of Peripheral Blood Mononuclear Cells. In many cases, PBMCs are derived from blood banks.
References
- Delves, Peter, et al. Roitt's Essential Immunology, 11th Ed. ISBN 978-1-4051-3603-7
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