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Revision as of 13:28, 3 December 2010 editCheMoBot (talk | contribs)Bots141,565 edits Updating {{chembox}} (no changed fields - added verified revid - updated 'UNII_Ref', 'ChemSpiderID_Ref', 'StdInChI_Ref', 'StdInChIKey_Ref') per Chem/Drugbox validation (report [[Wikipedia_talk:Wi← Previous edit Revision as of 03:36, 19 December 2012 edit undoLHcheM (talk | contribs)Extended confirmed users598 edits Added images of sample of methyl blue and its aqueous solution, with some cleanupNext edit →
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{{confuse|methyl violet|methylene blue}}

{{chembox {{chembox
| verifiedrevid = 400307025 | verifiedrevid = 400307025
| ImageFile = Methyl blue.png | ImageFile = Methyl blue.png
| ImageSize = | ImageSize = 220
| ImageFileL1 = Sample of Methyl Blue.jpg
| ImageSizeL1 = 130
| ImageCaptionL1 = Solid methyl blue
| ImageFileR1 = Methyl Blue aqueous solution.jpg
| ImageSizeR1 = 130
| ImageCaptionR1 = Methyl blue aqueous solution
| IUPACName = | IUPACName =
| OtherNames = | OtherNames = Cotton blue, Helvetia blue, Acid blue 93, C.I. 42780'''
| Section1 = {{Chembox Identifiers | Section1 = {{Chembox Identifiers
| ChemSpiderID_Ref = {{chemspidercite|correct|chemspider}} | ChemSpiderID_Ref = {{chemspidercite|correct|chemspider}}
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| Formula = C<sub>37</sub>H<sub>27</sub>N<sub>3</sub>Na<sub>2</sub>O<sub>9</sub>S<sub>3</sub> | Formula = C<sub>37</sub>H<sub>27</sub>N<sub>3</sub>Na<sub>2</sub>O<sub>9</sub>S<sub>3</sub>
| MolarMass = 799.814 g/mol | MolarMass = 799.814 g/mol
| Appearance = | Appearance = red solid
| Density = | Density =
| MeltingPt = | MeltingPt =
| BoilingPt = | BoilingPt =
| Solubility = ~ 300 g/l (20 °C) | Solubility = Soluble in water, slightly soluble in ethanol
}} }}
| Section3 = {{Chembox Hazards | Section3 = {{Chembox Hazards
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}} }}
}} }}
'''Methyl blue''', also known as '''Cotton blue''', '''Helvetia blue''', '''Acid blue 93''', or '''C.I. 42780''', is a ] with the ] C<sub>37</sub>H<sub>27</sub>N<sub>3</sub>Na<sub>2</sub>O<sub>9</sub>S<sub>3</sub>. It is used as a ] in ]. Methyl blue stains ] blue in ] sections. It is soluble in water and slightly soluble in ]. It can be used in the ] ] stain and ]. It is used in ]. It can also be used to mediate electron transfer in ]s. '''Methyl blue''' is a ] with the ] C<sub>37</sub>H<sub>27</sub>N<sub>3</sub>Na<sub>2</sub>O<sub>9</sub>S<sub>3</sub>. It is used as a ] in ], and stains ] blue in ] sections. It can be used in some ] techniques such as ] ] stain and ], and can be used to mediate electron transfer in ]s. Fungal cell walls are also stained by methyl blue.


Methyl blue is also used to stain fungal cell walls.
Methyl blue is also available in mixture with ], under name ], Aniline blue, China blue, or Soluble blue. Methyl blue is also available in mixture with ], under name ], Aniline blue, China blue, or Soluble blue.

Methyl blue should not be confused with ] or ], two other stains.

Methyl blue is also used to treat ] poisoning. It binds to the thallium.


==See also== ==See also==

Revision as of 03:36, 19 December 2012

Not to be confused with methyl violet or methylene blue.
Methyl blue
Solid methyl blue
Methyl blue aqueous solution
Names
Other names Cotton blue, Helvetia blue, Acid blue 93, C.I. 42780
Identifiers
CAS Number
3D model (JSmol)
ChemSpider
ECHA InfoCard 100.044.852 Edit this at Wikidata
PubChem CID
CompTox Dashboard (EPA)
InChI
  • InChI=1S/C37H29N3O9S3.2Na/c41-50(42,43)34-19-13-31(14-20-34)38-28-7-1-25(2-8-28)37(26-3-9-29(10-4-26)39-32-15-21-35(22-16-32)51(44,45)46)27-5-11-30(12-6-27)40-33-17-23-36(24-18-33)52(47,48)49;;/h1-24,38-39H,(H,41,42,43)(H,44,45,46)(H,47,48,49);;/q;2*+1/p-2Key: MCPLVIGCWWTHFH-UHFFFAOYSA-L
  • InChI=1/C37H29N3O9S3.2Na/c41-50(42,43)34-19-13-31(14-20-34)38-28-7-1-25(2-8-28)37(26-3-9-29(10-4-26)39-32-15-21-35(22-16-32)51(44,45)46)27-5-11-30(12-6-27)40-33-17-23-36(24-18-33)52(47,48)49;;/h1-24,38-39H,(H,41,42,43)(H,44,45,46)(H,47,48,49);;/q;2*+1/p-2Key: MCPLVIGCWWTHFH-NUQVWONBAI
SMILES
  • ..S(=O)(=O)c1ccc(cc1)Nc2ccc(cc2)/C(=C\4/C=C\C(=/c3ccc(cc3)S()(=O)=O)/C=C/4)c6ccc(Nc5ccc(cc5)S()(=O)=O)cc6
Properties
Chemical formula C37H27N3Na2O9S3
Molar mass 799.814 g/mol
Appearance red solid
Solubility in water Soluble in water, slightly soluble in ethanol
Hazards
NFPA 704 (fire diamond)
NFPA 704 four-colored diamondHealth 1: Exposure would cause irritation but only minor residual injury. E.g. turpentineFlammability 1: Must be pre-heated before ignition can occur. Flash point over 93 °C (200 °F). E.g. canola oilInstability 0: Normally stable, even under fire exposure conditions, and is not reactive with water. E.g. liquid nitrogenSpecial hazards (white): no code
1 1 0
Except where otherwise noted, data are given for materials in their standard state (at 25 °C , 100 kPa). checkY verify (what is  ?) Infobox references
Chemical compound

Methyl blue is a chemical compound with the molecular formula C37H27N3Na2O9S3. It is used as a stain in histology, and stains collagen blue in tissue sections. It can be used in some differential staining techniques such as Mallory's connective tissue stain and Gömöri trichrome stain, and can be used to mediate electron transfer in microbial fuel cells. Fungal cell walls are also stained by methyl blue.

Methyl blue is also available in mixture with water blue, under name Aniline Blue WS, Aniline blue, China blue, or Soluble blue.

See also


References

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Microbial and histological stains
Iron/hemosiderin
Lipids
Carbohydrates
Amyloid
Bacteria
Connective tissue
Other
Tissue stainability
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