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| {{Refimprove|date=December 2009}} | |||
| {{Infobox isotope | |||
| | alternate_names = | |||
| | symbol =N | |||
| | mass_number =15 | |||
| | mass = 15.000 108 898 4(9) | |||
| | num_neutrons =8 | |||
| | num_protons =7 | |||
| | abundance = 0.37% | |||
| | halflife = | |||
| | error_halflife = | |||
| googlearfglakdfjg | |||
| | text_color = | |||
| | image = | |||
| | decay_product = | |||
| | decay_symbol = | |||
| | decay_mass = | |||
| | decay_mode1 = | |||
| | decay_energy1 = | |||
| | decay_mode2 = | |||
| | decay_energy2 = | |||
| | decay_mode3 = | |||
| | decay_energy3 = | |||
| | decay_mode4 = | |||
| | decay_energy4 = | |||
| | parent =Oxygen-15 | |||
| | parent_symbol =O | |||
| | parent_mass =15 | |||
| | parent_decay =positron emission | |||
| | parent2 = | |||
| | parent2_symbol = | |||
| | parent2_mass = | |||
| | parent2_decay = | |||
| | spin = ½ | |||
| | excess_energy = | |||
| | error1 = | |||
| | binding_energy = | |||
| | error2 = | |||
| }} | |||
| '''Nitrogen-15''' is a stable, non-radioactive ] of ]. It is often used in ] and ] research, for example the ] to establish the nature of ].<ref>Meselson, M., Stahl, F.W., 1958. The replication of DNA in E. coli. Proc. Natl. Acad. Sci. USA 44, 671–682.</ref> An extension of this research resulted in development of DNA-based stable isotope probing, which allows examination of links between metabolic function and taxonomic identity of microorganisms in the environment, without the need for culture isolation.<ref>Radajewski, S., McDonald, I.R., Murrell, J.C., 2003. Stable-isotope probing of nucleic acids: a window to the function of uncultured microorganisms. Curr. Opin. Biotechnol. 14, 296–302.</ref><ref>Cupples, A.M., E.A. Shaffer, J.C. Chee-Sanford, and G.K. Sims. 2007. DNA buoyant density shifts during 15N DNA stable isotope probing. Microbiological Res. 162:328-334.</ref> Nitrogen-15 is extensively used to trace mineral nitrogen compounds (particularly fertilizers) in the environment and when combined with the use of other isotopic labels, is also a very important tracer for describing the fate of nitrogenous organic pollutants.<ref>Marsh, K. L., G. K. Sims, and R. L. Mulvaney. 2005. Availability of urea to autotrophic ammonia-oxidizing bacteria as related to the fate of 14C- and 15N-labeled urea added to soil. Biol. Fert. Soil. 42:137-145.</ref><ref>Bichat, F., G.K. Sims, and R.L. Mulvaney. 1999. Microbial utilization of heterocyclic nitrogen from atrazine. Soil Sci. Soc. Am. J. 63:100-110.</ref> | |||
| Nitrogen-15 is frequently used in ] (NMR), because unlike the more abundant ], it has a ] ] of ½, which simplifies observation by NMR. ]s can be isotopically labelled by cultivating them in a medium containing nitrogen-15 as the only source of nitrogen. In addition, nitrogen-15 is used to label proteins in quantitative ] (e.g. ]). | |||
| It is a product of ] ].<ref>CRC HANDBOOK of CHEMISTRY and PHYSICS, 64 th EDITION, 1983-1984; page B-234</ref> | |||
| == Reference == | |||
| {{Reflist}} | |||
| ⚫ | ] | ||
| ] | |||
| {{Isotope-stub}} | |||
| {{Nuclear-stub}} | |||
| ] | |||
| ] | |||
Revision as of 11:17, 27 March 2011
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