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'''Acid fuchsin''' or '''fuchsine acid''', (also called '''Acid Violet 19'''<ref name="Lillie, 1977" /> and '''] 42685'''<ref name="Lillie, 1977" />) is an ]ic ] ] with the ] C<sub>20</sub>H<sub>17</sub>N<sub>3</sub>Na<sub>2</sub>O<sub>9</sub>S<sub>3</sub>. Acid fuchsin has wide use in ],<ref name="Lillie, 1977" /> and is one of the dyes used in ].<ref>Jocelyn H. Bruce-Gregorios, M.D.: Histopathologic Techniques, JMC Press Inc., Quezon City, Philippines, 1974. {{ISBN|971-11-0853-4}}</ref> This method is commonly used to stain ] and nuclei of tissue sections in the histology laboratory in order to distinguish muscle from ]. The muscle stains red with the acid fuchsin, and the collagen is stained green or blue with ] or ]. It can also be used to identify growing bacteria.<ref>{{cite journal|title=The use of Decolorized Acid Fuchsin as an Acid Indicator in Carbohydrate Fermentation Tests with some Remarks on Acid Production by Bacteria|journal=Journal of Infectious Diseases|volume=15|pages=227–233|doi=10.1093/infdis/15.1.227|year=1914|last1=Holman|first1=W. L|url=https://zenodo.org/record/2508513}}</ref> '''Acid fuchsin''' or '''fuchsine acid''', (also called '''Acid Violet 19'''<ref name="Lillie, 1977" /> and '''] 42685'''<ref name="Lillie, 1977" />) is an ]ic ] ] with the ] C<sub>20</sub>H<sub>17</sub>N<sub>3</sub>Na<sub>2</sub>O<sub>9</sub>S<sub>3</sub>. It is a sodium ] derivative of ]. Acid fuchsin has wide use in ],<ref name="Lillie, 1977" /> and is one of the dyes used in ].<ref>Jocelyn H. Bruce-Gregorios, M.D.: Histopathologic Techniques, JMC Press Inc., Quezon City, Philippines, 1974. {{ISBN|971-11-0853-4}}</ref> This method is commonly used to stain ] and nuclei of tissue sections in the histology laboratory in order to distinguish muscle from ]. The muscle stains red with the acid fuchsin, and the collagen is stained green or blue with ] or ]. It can also be used to identify growing bacteria.<ref>{{cite journal|title=The use of Decolorized Acid Fuchsin as an Acid Indicator in Carbohydrate Fermentation Tests with some Remarks on Acid Production by Bacteria|journal=Journal of Infectious Diseases|volume=15|pages=227–233|doi=10.1093/infdis/15.1.227|year=1914|last1=Holman|first1=W. L|url=https://zenodo.org/record/2508513}}</ref>


== See also == == See also ==
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Latest revision as of 13:33, 12 March 2022

Acid fuchsin
Names
IUPAC name Disodium 2-amino-5--3-methylbenzenesulfonate
Identifiers
CAS Number
3D model (JSmol)
ChEBI
ChemSpider
ECHA InfoCard 100.019.833 Edit this at Wikidata
EC Number
  • 221-816-5
PubChem CID
UNII
CompTox Dashboard (EPA)
InChI
  • InChI=1S/C20H19N3O9S3.2Na/c1-10-6-13(9-18(20(10)23)35(30,31)32)19(11-2-4-14(21)16(7-11)33(24,25)26)12-3-5-15(22)17(8-12)34(27,28)29;;/h2-9,21H,22-23H2,1H3,(H,24,25,26)(H,27,28,29)(H,30,31,32);;/q;2*+1/p-2/b19-11-,21-14?;;Key: RZUBARUFLYGOGC-MTHOTQAESA-L
  • InChI=1/C20H19N3O9S3.2Na/c1-10-6-13(9-18(20(10)23)35(30,31)32)19(11-2-4-14(21)16(7-11)33(24,25)26)12-3-5-15(22)17(8-12)34(27,28)29;;/h2-9,21H,22-23H2,1H3,(H,24,25,26)(H,27,28,29)(H,30,31,32);;/q;2*+1/p-2/b19-11-,21-14?;;Key: RZUBARUFLYGOGC-LOQRZINWBG
SMILES
  • CC1=CC(=CC(=C1N)S(=O)(=O))/C(=C\2/C=CC(=)C(=C2)S(=O)(=O))/C3=CC(=C(C=C3)N)S(=O)(=O)..
Properties
Chemical formula C20H17N3Na2O9S3
Molar mass 585.53 g·mol
Hazards
GHS labelling:
Pictograms GHS07: Exclamation mark
Signal word Warning
Hazard statements H315, H319, H335
Precautionary statements P261, P264, P271, P280, P302+P352, P304+P340, P305+P351+P338, P312, P321, P332+P313, P337+P313, P362, P403+P233, P405, P501
Except where otherwise noted, data are given for materials in their standard state (at 25 °C , 100 kPa). checkY verify (what is  ?) Infobox references
Chemical compound

Acid fuchsin or fuchsine acid, (also called Acid Violet 19 and C.I. 42685) is an acidic magenta dye with the chemical formula C20H17N3Na2O9S3. It is a sodium sulfonate derivative of fuchsine. Acid fuchsin has wide use in histology, and is one of the dyes used in Masson's trichrome stain. This method is commonly used to stain cytoplasm and nuclei of tissue sections in the histology laboratory in order to distinguish muscle from collagen. The muscle stains red with the acid fuchsin, and the collagen is stained green or blue with Light Green SF yellowish or methyl blue. It can also be used to identify growing bacteria.

See also

References

  1. ^ Lillie, Ralph Dougall (1977). H. J. Conn's Biological stains (9th ed.). Baltimore: Williams & Wilkins. p. 692.
  2. Jocelyn H. Bruce-Gregorios, M.D.: Histopathologic Techniques, JMC Press Inc., Quezon City, Philippines, 1974. ISBN 971-11-0853-4
  3. Holman, W. L (1914). "The use of Decolorized Acid Fuchsin as an Acid Indicator in Carbohydrate Fermentation Tests with some Remarks on Acid Production by Bacteria". Journal of Infectious Diseases. 15: 227–233. doi:10.1093/infdis/15.1.227.
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