Misplaced Pages

Peripheral blood mononuclear cell: Difference between revisions

Article snapshot taken from Wikipedia with creative commons attribution-sharealike license. Give it a read and then ask your questions in the chat. We can research this topic together.
Browse history interactively← Previous editNext edit →Content deleted Content addedVisualWikitext
Revision as of 22:36, 16 July 2010 editWLU (talk | contribs)Autopatrolled, Extended confirmed users, Pending changes reviewers, Rollbackers52,243 edits name← Previous edit Revision as of 12:41, 1 August 2010 edit undoCallmederek (talk | contribs)345 edits moving cat to redirect pageNext edit →
Line 15: Line 15:
{{DEFAULTSORT:Pbmc}} {{DEFAULTSORT:Pbmc}}
] ]
]


==References== ==References==

Revision as of 12:41, 1 August 2010

For the internet slang term "PBMC", see ProblemBetweenMonitorChair.
It has been suggested that this article be merged into Agranulocyte. (Discuss) Proposed since February 2010.

A peripheral blood mononuclear cell (PBMC) is any blood cell having a round nucleus. For example: a lymphocyte, a monocyte or a macrophage. These blood cells are a critical component in the immune system to fight infection and adapt to intruders. The lymphocyte population consists of T cells (CD4 and CD8 positive ~75%), B cells and NK cells (~25% combined).

These cells are often extracted from whole blood using ficoll, a hydrophilic polysaccharide that separates layers of blood, with monocytes and lymphocytes forming a buffy coat under a layer of plasma. This buffy coat contains the PBMCs. Additionally, PBMC can be extracted from whole blood using a hypotonic lysis which will preferentially lyse red blood cells. This method results in neutrophils and other polymorphonuclear (PMN) cells which are important in innate immune defence being obtained.

PBMCs are widely used in research and clinical uses every day. HIV research uses them because PBMCs include CD4+ cells, which are the cells HIV infects.

See also Peripheral blood cell.

Research Applications

Many scientists conducting research in the fields of immunology (including auto-immune disorders), infectious disease, hematological malignancies, vaccine development, Transplant Immunology, and high-throughput screening are frequent users of Peripheral Blood Mononuclear Cells. In many cases, PBMCs are derived from blood banks.

References

  1. Delves, Peter, et al. Roitt's Essential Immunology, 11th Ed. ISBN: 978-1-4051-3603-7
Stub icon

This cell biology article is a stub. You can help Misplaced Pages by expanding it.

Stub icon

This immunology article is a stub. You can help Misplaced Pages by expanding it.

Categories: