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Defending champion Serena Williams defeated her sisterVenus Williams, the two-time defending champion, in a rematch of the previous year's final, 4–6, 6–4, 6–2 to win the ladies' singles tennis title at the 2003 Wimbledon Championships. It was her second Wimbledon singles title and her sixth major singles title overall.
This was the first Wimbledon appearance of future world No. 1 and five-time major champion Maria Sharapova, who lost to Svetlana Kuznetsova in the fourth round. Sharapova would win the title the following year. It was also the first Wimbledon appearance of future champion Marion Bartoli, who lost to Daniela Hantuchová in the first round. The semifinals featured the top four seeds, which last occurred in 1995.
Click on the seed number of a player to go to their draw section.
Amélie Mauresmo withdrew due to a rib injury. She was replaced in the draw by the highest-ranked non-seeded player Svetlana Kuznetsova, who became the #33 seed.