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Cresyl violet

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Cresyl violet
Names
IUPAC name (9-dimethylamino-10-methyl-benzophenoxazin-5-ylidene)ammonium chloride
Other names 9-(Dimethylamino)-5-imino-10-methyl-5H-benzo(a)phenoxazine hydrochloride; Cresole Violet
Identifiers
3D model (JSmol)
ChemSpider
CompTox Dashboard (EPA)
InChI
  • InChI=1S/C19H17N3O.ClH/c1-11-8-15-17(10-16(11)22(2)3)23-18-9-14(20)12-6-4-5-7-13(12)19(18)21-15;/h4-10,20H,1-3H3;1HKey: ZHAFUINZIZIXFC-UHFFFAOYSA-N
  • InChI=1/C19H17N3O.ClH/c1-11-8-15-17(10-16(11)22(2)3)23-18-9-14(20)12-6-4-5-7-13(12)19(18)21-15;/h4-10,20H,1-3H3;1HKey: ZHAFUINZIZIXFC-UHFFFAOYAN
SMILES
  • .N=1c4c(OC=3C=1c2ccccc2\C(=)\C=3)cc(c(c4)C)N(C)C
Properties
Chemical formula C19H18ClN3O
Molar mass 339.8187
Hazards
Flash point 245.5 °C
Except where otherwise noted, data are given for materials in their standard state (at 25 °C , 100 kPa). checkverify (what is  ?) Infobox references
Chemical compound

Cresyl violet is an organic compound with the chemical formula C19H18ClN3O. It is used in biology and medicine as a histological stain. Cresyl violet is an effective and reliable stain used for light microscopy sections. Initially, tissue sections are ‘defatted’ by passing through graded dilutions of ethanol. Then, rehydrated by passing back through decreasing concentrations of ethanol. Lastly, back into water. The ethanol solutions act to differentiate the stain, causing myelin and other components to lose color whereas perikarya retain the color.


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