| Revision as of 12:50, 6 December 2011 editBeetstra (talk | contribs)Edit filter managers, Administrators172,031 edits Saving copy of the {{chembox}} taken from revid 415981960 of page Rhodamine_123 for the Chem/Drugbox validation project (updated: 'ChEMBL', 'CASNo'). |
Latest revision as of 01:47, 24 February 2021 edit LegionMammal978 (talk | contribs)Extended confirmed users7,894 editsm corrected IUPAC name |
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{{ambox | text = This page contains a copy of the infobox ({{tl|chembox}}) taken from revid of page ] with values updated to verified values.}} |
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| verifiedrevid = 401041023 |
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|ImageFile=Rhodamine 123.svg |
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| verifiedrevid = 464381696 |
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| ImageFile =Rhodamine 123.svg |
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|IUPACName=6-amino-9-(2-methoxycarbonylphenyl)xanthen-3-ylidene]azanium chloride |
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|OtherNames=Rhodamine 123, EINECS 263-687-8, RH 123, LS-162564, C11190 |
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| PIN = 7-Amino-10--2''H''-xanthene-2-iminium chloride |
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| OtherNames =Rhodamine 123, EINECS 263-687-8, RH 123, LS-162564, C11190 |
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|Section1={{Chembox Identifiers |
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|Section1={{Chembox Identifiers |
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| ChemSpiderID_Ref = {{chemspidercite|correct|chemspider}} |
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| ChemSpiderID_Ref = {{chemspidercite|correct|chemspider}} |
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| ChemSpiderID = 13726662 |
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| ChemSpiderID = 13726662 |
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| InChI = 1/C21H17N2O3.ClH/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20;/h2-11H,22-23H2,1H3;1H/q+1;/p-1 |
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| InChI = 1/C21H17N2O3.ClH/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20;/h2-11H,22-23H2,1H3;1H/q+1;/p-1 |
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| StdInChIKey_Ref = {{stdinchicite|correct|chemspider}} |
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| StdInChIKey_Ref = {{stdinchicite|correct|chemspider}} |
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| StdInChIKey = TUFFYSFVSYUHPA-UHFFFAOYSA-M |
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| StdInChIKey = TUFFYSFVSYUHPA-UHFFFAOYSA-M |
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| CASNo_Ref = {{cascite|correct|CAS}} |
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| CASNo = <!-- blanked - oldvalue: 62669-70-9 --> |
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| CASNo =62669-70-9 |
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| ChEMBL = 245839 |
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| UNII_Ref = {{fdacite|correct|FDA}} |
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| UNII = 1N3CZ14C5O |
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| ChEMBL_Ref = {{ebicite|changed|EBI}} |
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| ChEMBL = 176049 |
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| EC_number = 263-687-8 |
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| PubChem = 9929799 |
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|Section2={{Chembox Properties |
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| Formula=C<sub>21</sub>H<sub>17</sub>ClN<sub>2</sub>O<sub>3</sub> |
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| Formula =C<sub>21</sub>H<sub>17</sub>ClN<sub>2</sub>O<sub>3</sub> |
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| MolarMass=380.824 |
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| MolarMass =380.824 |
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| MeltingPtC = 235 |
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| MeltingPt=235 °C |
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| Solvent = ] |
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| Solvent = ] |
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| SolubleOther = 20 g/l |
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| SolubleOther = 20 g/l |
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|Section3={{Chembox Hazards |
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| GHSPictograms = {{GHS07}} |
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| GHSSignalWord = Warning |
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| HPhrases = {{H-phrases|302}} |
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| PPhrases = {{P-phrases|264|270|301+312|330|501}} |
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'''Rhodamine 123''' {{IPAc-en|ˈ|r|oʊ|d|əm|iː|n}} is a chemical compound and a ]. It is often used as a ] within water to determine the rate and direction of flow and transport. Rhodamine dyes ] and can thus be detected easily and inexpensively with instruments called ]s. Rhodamine dyes are used extensively in biotechnology applications such as ], ], ] and ]. Rhodamine fluorescence can also be used as a measure of membrane polarization in live cell assays both within mitochondria<ref>L. B. Chen. "Mitochondrial membrane potential in living cells."</ref><ref>Darzynkiewicz Z, Traganos F, Staiano-Coico L, Kapuscinski J, Melamed MR. (1982) Interaction of rhodamine 123 with living cells studied by flow cytometry. Cancer Res. Mar;42(3):799-806. {{PMID|7059978}}</ref> and with bacteria. This use relies on the fact that rhodamine 123 accumulates in membranes in a manner which is dependent on membrane polarization.<ref>M. Huang, A. K. S. Camara, D. F. Stowe, F. Qi, D. A. Beard. "Mitochondrial inner membrane electrophysiology assessed by rhodamine-123 transport and fluorescence" </ref> |
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The absorption of rhodamine 123 peaks around 505 nm and luminescence is tunable around 560 nm when used as a ].<ref></ref> Its luminescence ] is 0.90.<ref>R. F. Kubin and A. N. Fletcher, "Fluorescence quantum yields of some rhodamine dyes." </ref> |
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==References== |
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{{reflist}} |
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==See also== |
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*]s |
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{{Dye laser}} |
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