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Eight UCI WorldTeams, twelve UCI ProTeams, and three UCI Continental teams made up the twenty-three teams that participated the race. Each team entered six riders, except for Leopard Pro Cycling, which entered five, for a starting peloton of 138 riders. 103 of these riders finished the race.
16 September 2020 — Remich to Hesperange, 160.8 km (99.9 mi) 40.7 km (25.3 mi)
Riders held a protest during the opening kilometers of the stage to voice several of their safety concerns about the previous stage. They pointed out that there were parked vehicles, as well as a bus that was stopped on the road with under five kilometers to go. Lithuanian rider Ignatas Konovalovas of Groupama–FDJ had even found himself having to stop for a red light towards the end of that stage when the roads were opened back up to traffic prematurely. Riders had spoken to race organizers about these hazards before stage 2, but when they encountered further problems on stage 2, they stopped. It was only after negotiations with race organizers and UCI did the riders agree to resume, but only after neutralizing the 80 kilometer route to Syren and only resume racing in the final 42 kilometers in the circuit around Hesperange.