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ADA (buffer)

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ADA
Chemical structure of ADA
Names
Systematic IUPAC name 2,2′-diacetic acid
Other names ADA, N-(2-acetamido)iminodiacetic acid, N-(carbamoylmethyl)iminodiacetic acid
Identifiers
CAS Number
3D model (JSmol)
ChEBI
ChemSpider
DrugBank
ECHA InfoCard 100.043.194 Edit this at Wikidata
EC Number
  • 247-530-0
PubChem CID
UNII
CompTox Dashboard (EPA)
InChI
  • InChI=1S/C6H10N2O5/c7-4(9)1-8(2-5(10)11)3-6(12)13/h1-3H2,(H2,7,9)(H,10,11)(H,12,13)Key: QZTKDVCDBIDYMD-UHFFFAOYSA-N
SMILES
  • C(C(=O)N)N(CC(=O)O)CC(=O)O
Properties
Chemical formula C6H10N2O5
Molar mass 190.155 g/mol
Acidity (pKa) 6.6
Hazards
Occupational safety and health (OHS/OSH):
Main hazards Irritant
GHS labelling:
Signal word Warning
Hazard statements H302, H315, H319, H335
Precautionary statements P261, P264, P270, P271, P280, P301+P312, P302+P352, P304+P340, P305+P351+P338, P312, P321, P330, P332+P313, P337+P313, P362, P403+P233, P405, P501
Flash point Non-flammable
Except where otherwise noted, data are given for materials in their standard state (at 25 °C , 100 kPa). Infobox references
Chemical compound

ADA is a zwitterionic organic chemical buffering agent; one of Good's buffers. It has a useful pH range of 6.0-7.2 in the physiological range, making it useful for cell culture work. It has a pKa of 6.6 with ΔpKa/°C of -0.011 and is most often prepared in 1 M NaOH where it has a solubility of 160 mg/mL.

ADA has been used in protein-free media for chicken embryo fibroblasts, as a chelating agent for H, Ca, and Mg, and for isoelectric focusing in immobilized pH gradients. Its effects on dog kidney Na/K-ATPase and rat brain GABA receptors have also been studied. ADA does, however, alter coloring in bicinchoninic acid assays.

References

  1. "ADA" (PDF). Sigma-Aldrich.
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