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Amido black 10B

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Amido black 10B
Names
IUPAC name Sodium 4-amino-5-hydroxy-3-((E)-(4-nitrophenyl)diazenyl)-6-((E)-phenyldiazenyl)naphthalene-2,7-disulfonate
Other names 4-Amino-5-hydroxy-3--6-(phenylazo)-2,7-naphthalene disulfonic acid, disodium salt
Amidoschwarz
Naphthol blue black
Acid Black 1
Acidal Black 10B
Acidal Navy Blue 3BR
Naphthalene Black 10B
Buffalo Black NBR
C.I. 20470
Identifiers
CAS Number
3D model (JSmol)
ChEBI
ChemSpider
ECHA InfoCard 100.012.640 Edit this at Wikidata
EC Number
  • 213-903-1
PubChem CID
UNII
CompTox Dashboard (EPA)
InChI
  • InChI=1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;Key: AOMZHDJXSYHPKS-DROYEMJCSA-L
  • InChI=1/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;Key: AOMZHDJXSYHPKS-NJAWIMKDBA
SMILES
  • ..O=N(=O)c1ccc(cc1)/N=N/c2c(N)c4c(cc2S()(=O)=O)cc(c(/N=N/c3ccccc3)c4O)S()(=O)=O
Properties
Chemical formula C22H14N6Na2O9S2
Molar mass 616.49 g·mol
Appearance Dark red to black
Density 1.536 g/cm
Solubility in water ~ 30 g/L at 20 °C (68 °F)
Hazards
GHS labelling:
Pictograms GHS07: Exclamation mark
Signal word Warning
NFPA 704 (fire diamond)
NFPA 704 four-colored diamondHealth 2: Intense or continued but not chronic exposure could cause temporary incapacitation or possible residual injury. E.g. chloroformFlammability 0: Will not burn. E.g. waterInstability 0: Normally stable, even under fire exposure conditions, and is not reactive with water. E.g. liquid nitrogenSpecial hazards (white): no code
2 0 0
Except where otherwise noted, data are given for materials in their standard state (at 25 °C , 100 kPa). ☒N verify (what is  ?) Infobox references
Chemical compound

Amido black 10B is an amino acid staining azo dye used in biochemical research to stain for total protein on transferred membrane blots, such as the western blot. It is also used in criminal investigations to detect blood present with latent fingerprints - it stains the proteins in blood a blue-black color. Amido Black can be either methanol or water based as it readily dissolves in both. With picric acid, in a van Gieson procedure, it can be used to stain collagen and reticulin.

See also

References

  1. "AMIDO BLACK 10B". chemicalland21.com. Retrieved December 2, 2017.
  2. Kurien, B. T., & Scofield, R. H. (2015). Western Blotting: An Introduction. In B. T. Kurien & R. H. Scofield (Eds.), Western Blotting: Methods and Protocols (pp. 17–30). Springer. ISBN 978-1-4939-2694-7
  3. Bossers, L. C. A. M., Roux, C., Bell, M., & McDonagh, A. M. (2011). Methods for the enhancement of fingermarks in blood. Forensic Science International, 210(1), 1–11. doi:10.1016/j.forsciint.2011.04.006

External links

Glutamate metabolism and transport modulators
Transporter
EAATsTooltip Excitatory amino acid transporters
vGluTsTooltip Vesicular glutamate transporters
Enzyme
GAHTooltip Glutamine aminohydrolase (glutaminase)
ASTTooltip Aspartate aminotransferase
ALTTooltip Alanine aminotransferase
GDHTooltip Glutamate dehydrogenase
GSTooltip Glutamine synthetase
GADTooltip Glutamate decarboxylase
See also: Receptor/signaling modulators • Ionotropic glutamate receptor modulators • Metabotropic glutamate receptor modulators • GABA metabolism and transport modulators
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