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Cresol Red

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Cresol Red
Names
Preferred IUPAC name 3,3-Bis(4-hydroxy-3-methylphenyl)-2,1λ-benzoxathiole-1,1(3H)-dione
Identifiers
CAS Number
3D model (JSmol)
ChEBI
ChemSpider
ECHA InfoCard 100.015.513 Edit this at Wikidata
PubChem CID
UNII
CompTox Dashboard (EPA)
InChI
  • InChI=1S/C21H18O5S/c1-13-11-15(7-9-18(13)22)21(16-8-10-19(23)14(2)12-16)17-5-3-4-6-20(17)27(24,25)26-21/h3-12,22-23H,1-2H3Key: OBRMNDMBJQTZHV-UHFFFAOYSA-N
  • InChI=1/C21H18O5S/c1-13-11-15(7-9-18(13)22)21(16-8-10-19(23)14(2)12-16)17-5-3-4-6-20(17)27(24,25)26-21/h3-12,22-23H,1-2H3Key: OBRMNDMBJQTZHV-UHFFFAOYAD
SMILES
  • Cc1cc(ccc1O)/C(=C/2C=CC(=O)C(=C2)C)c3ccccc3OS(O)=O
Properties
Chemical formula C21H17NaO5S
Molar mass 382.43 g/mol
Except where otherwise noted, data are given for materials in their standard state (at 25 °C , 100 kPa). ☒N verify (what is  ?) Infobox references
Chemical compound

Cresol red (full name: o-cresolsulfonephthalein) is a triarylmethane dye frequently used for monitoring the pH in aquaria.

Cresol red (pH indicator)
below pH 7.2 above pH 8.8
7.2 ⇌ 8.8

Molecular biology

Cresol red can be used in many common molecular biology reactions in place of other loading dyes. Cresol Red does not inhibit Taq polymerase to the same degree as other common loading dyes.

Color marker

Cresol red can also be used as an electrophoretic color marker to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis. In a 1% agarose gel, it runs approximately at the size of a 125 base pair (bp) DNA molecule (it depends on the concentration of buffer and other component). Bromophenol blue and xylene cyanol can also be used for this purpose.

References

  1. PubChem. "Cresol red". pubchem.ncbi.nlm.nih.gov. Retrieved 2023-01-25.

External links

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