Article snapshot taken from Wikipedia with creative commons attribution-sharealike license.
Give it a read and then ask your questions in the chat.
We can research this topic together.
Deoxyribonuclease II (EC3.1.22.1, DNase II, pancreatic DNase II, deoxyribonucleate 3'-nucleotidohydrolase, pancreatic DNase II, acid deoxyribonuclease, acid DNase) is an endonuclease that hydrolyzes phosphodiester linkages of deoxyribonucleotide in native and denatured DNA, yielding products with 3'-phosphates and 5'-hydroxyl ends, which occurs as a result of single-strand cleaving mechanism. As the name implies, it functions optimally at acidpH because it is commonly found in low pH environment of lysosomes.
The action of DNase occurs in three phases. The initial phase introduces multiple nicks in the phosphodiester backbone. The second phase produces acid-soluble nucleotides. The third phase, which is the terminal phase, consists of hyperchromic shift resulting from reduction of oligonucleotides.
DNase II alpha (usually known as DNase II), which is thought to be ubiquitously expressed in human tissue. It has been shown that a mutation in this enzyme of mice leads to DNA degradation by apoptosis.
DNase II beta (also called DLAD, or DNase II-Like Acid DNase), which is mainly expressed in the eye lens and salivary glands. One of its functions is to clear DNA from eye lens. Low levels have also been detected in the lung, prostate and lymph nodes. Deficiency of this enzyme in mice lead to the development of cataracts.
References
^ Bernardi G (1971). "Chapter 11: Spleen Acid Deoxyribonuclease". In Boyer PD (ed.). The Enzymes. Vol. 4 (3rd ed.). New York: Elsevier Science. pp. 271–287. doi:10.1016/S1874-6047(08)60371-6. ISBN978-0-12-122704-3.
Universal protein resource accession number Q8WZ79 for "DNASE2B – Deoxyribonuclease-2-beta precursor – Homo sapiens (Human) – DNASE2B gene & protein" at UniProt.
