Day One may refer to:
Film and television
- Day One (1989 film), a 1989 television film
- Day One, also known as To Write Love on Her Arms, a 2012 drama film
- Day One (2015 film), a 2015 short film
- Day One (TV series), a 2009 American television pilot for NBC which was reduced from a television series to a television film, but which never aired
- Day One (TV program), an American news magazine program that ran from 1993 to 1995 on ABC
- "Day One" (Torchwood), a 2006 episode of the science-fiction television series Torchwood
- The first episode of the 2009 Torchwood serial Children of Earth
- Day One, the first season of the television series 24
- Day One, the second episode of the first season of the medical drama ER
- WWE Day 1, a 2022 professional wrestling event
Music
- Day One (band), an English trip hop band
- Day One (Birds of Tokyo album), a 2007 album by Birds of Tokyo
- Day One (From Ashes To New album), a 2016 album by From Ashes To New
- Day One (Sarah Slean album), a 2004 album by Sarah Slean
- Day One (Snob Scrilla album), a 2009 album by Snob Scrilla
- Day 1 (album), a 1991 album by Robbie Nevil
- "Day 1" (song), a song by Leslie Grace
- "Day One", a 2015 song by Chris Brown from Royalty
- "Day One", a 2011 song by Lights from Siberia
Video games
- Half-Life: Day One, a Half-Life demo
- Day One: Garry's Incident, a video game by Wild Games Studio
- Day 1 Studios, an American videogame developer
Other
- Day One (app), a personal journaling app for iOS, macOS and Android
- Day One (building), part of Amazon.com's downtown Seattle corporate headquarters
- Day One Christian Ministries, an organisation promoting Sunday as a day of rest, formerly known as the Lord's Day Observation Society (LDOS)
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