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Endo agar (also called Endo's medium) is a microbiological growth medium with a faint pink colour. Originally developed for the isolation of Salmonella typhi, it is now used mostly as a coliform medium. Most gram-negative organisms grow well in this medium, while growth of gram-positive organisms is inhibited. Coliform organisms ferment the lactose in this medium, producing a green metallic sheen (i.e. Escherichia coli), whereas non-lactose-fermenting organisms produce clear, colourless colonies, i.e. Salmonella species.

Typical composition

Endo agar typically contains (w/v):

• 1.0 % peptone
• 0.25 % dipotassium hydrogen phosphate (K₂HPO₄)
• 1.0 % lactose
• 0.33 % anhydrous sodium sulfite (Na₂SO₃)
• 0.03 % fuchsine
• 1.25 % agar

References

1. ^ "Mondofacto". Archived from the original on March 3, 2016. Retrieved April 25, 2009.
2. "Traditional Microbiology". Becton, Dickinson and Co. (BD). 2006. Archived from the original on March 3, 2016. Retrieved November 28, 2022.
3. EMD Chemicals, "ENDO Agar". 2002. Archived from the original on April 13, 2009. Retrieved April 25, 2009.

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