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Germacradienol synthase (EC 4.2.3.22, germacradienol/germacrene-D synthase, 2-trans,6-trans-farnesyl-diphosphate diphosphate-lyase ) is an enzyme with systematic name (2E,6E)-farnesyl-diphosphate diphosphate-lyase ((1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol-forming). This enzyme catalyses the following chemical reaction

(2E,6E)-farnesyl diphosphate + H₂O ${\displaystyle \rightleftharpoons }$ (1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + diphosphate

This enzyme requires Mg for activity.

References

1. Cane DE, Watt RM (February 2003). "Expression and mechanistic analysis of a germacradienol synthase from Streptomyces coelicolor implicated in geosmin biosynthesis". Proceedings of the National Academy of Sciences of the United States of America. 100 (4): 1547–51. doi:10.1073/pnas.0337625100. PMC 149869. PMID 12556563.
2. He X, Cane DE (March 2004). "Mechanism and stereochemistry of the germacradienol/germacrene D synthase of Streptomyces coelicolor A3(2)". Journal of the American Chemical Society. 126 (9): 2678–9. doi:10.1021/ja039929k. PMID 14995166.
3. Gust B, Challis GL, Fowler K, Kieser T, Chater KF (February 2003). "PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin". Proceedings of the National Academy of Sciences of the United States of America. 100 (4): 1541–6. doi:10.1073/pnas.0337542100. PMC 149868. PMID 12563033.

External links

• Germacradienol+synthase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

• Biology

• EC 4.2.3