Grey sisters may refer to:
- Graeae or Grey sisters, three old women from Greek mythology
- Lady Jane Grey, Lady Katherine Grey, and Lady Mary Grey from 16th-century Tudor England
- Meredith Grey, Lexie Grey and Molly Grey-Thompson from the TV series Grey's Anatomy
Catholic religious sisters
- Daughters of Charity of Saint Vincent de Paul or Grey sisters, society of apostolic life based in France
- Grey Nuns, Canadian religious institute based in Montreal
- Grey Sisters of the Immaculate Conception based in Pembroke, Ontario, Canada
- Family Care Sisters or Grey sisters, Australian congregation founded by Cecily Maude O'Connell
See also
- The Grey Sisterhood, 1916 American silent film
- Grey Sister, 2018 novel by Mark Lawrence
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