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Escherichia virus HK97

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(Redirected from HK97) Species of virus "HK97" redirects here. For other uses, see Hong Kong 97.
Escherichia virus HK97
Virus classification Edit this classification
(unranked): Virus
Realm: Duplodnaviria
Kingdom: Heunggongvirae
Phylum: Uroviricota
Class: Caudoviricetes
Order: Caudovirales
Family: Siphoviridae
Genus: Hendrixvirus
Species: Escherichia virus HK97

Escherichia virus HK97, often shortened to HK97, is a species of virus that infects Escherichia coli and related bacteria. It is named after Hong Kong (HK), where it was first located. HK97 has a double-stranded DNA genome.

Assembly and maturation

Escherichia virus HK97 as seen in transmission electron microscopy (TEM), with magnification 300000x.

The major capsid protein of HK97, called gp5, cross-links upon maturation to form a chain-mail like structure. While DNA is being packaged into the capsid, the capsid expands by nearly 5 nm and changes from spherical to icosahedral in shape.

The HK97 assembly pathway begins with self-assembly of gp5 into pentamers and hexamers. A protease, called gp4, cleaves gp5 at its N-terminus. Attachment of a portal protein, gp3, coupled with conformational changes leads to the formation of a prohead, or procapsid, which is the precursor to the mature capsid. A scaffolding protein is not required for capsid assembly. However, studies on the effects of deleting the delta domain of the major capsid protein, or parts of it, indicate that it is essential for assembly.

References

  1. Helgstrand, Charlotte; Wikoff, William R; Duda, Robert L; Hendrix, Roger W; Johnson, John E; Liljas, Lars (1 December 2003). "The Refined Structure of a Protein Catenane: The HK97 Bacteriophage Capsid at 3.44Å Resolution". Journal of Molecular Biology. 334 (5): 885–899. doi:10.1016/j.jmb.2003.09.035. PMID 14643655.
  2. Hendrix, RW; Johnson, JE (2012). "Bacteriophage HK97 Capsid Assembly and Maturation". Viral Molecular Machines. Advances in Experimental Medicine and Biology. Vol. 726. pp. 351–63. doi:10.1007/978-1-4614-0980-9_15. ISBN 978-1-4614-0979-3. PMID 22297521.
  3. Duda RL, Martincic K, Hendrix RW (1995). "Genetic basis of bacteriophage HK97 prohead assembly". J. Mol. Biol. 247 (4): 636–47. doi:10.1006/jmbi.1994.0169. PMID 7723020.
  4. Oh B, Moyer CL, Hendrix RW, Duda RL (2014). "The delta domain of the HK97 major capsid protein is essential for assembly". Virology. 456–457: 171–8. doi:10.1016/j.virol.2014.03.022. PMC 4044616. PMID 24889236.

Further reading

External links

Taxon identifiers
Enterobacteria phage HK97
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