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Bisbenzimide

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(Redirected from Hoechst 33342)
Bisbenzimide
Names
Preferred IUPAC name 2′-(4-Ethoxyphenyl)-6-(4-methylpiperazin-1-yl)-1H,3′H-2,5′-bi-1,3-benzimidazole
Other names Hoechst 33342; Hoe 33342
Identifiers
CAS Number
3D model (JSmol)
ChemSpider
ECHA InfoCard 100.041.523 Edit this at Wikidata
PubChem CID
UNII
CompTox Dashboard (EPA)
InChI
  • InChI=1S/C27H28N6O/c1-3-34-21-8-4-18(5-9-21)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-7-20(17-25(23)31-27)33-14-12-32(2)13-15-33/h4-11,16-17H,3,12-15H2,1-2H3,(H,28,30)(H,29,31)Key: PRDFBSVERLRRMY-UHFFFAOYSA-N
  • InChI=1/C27H28N6O/c1-3-34-21-8-4-18(5-9-21)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-7-20(17-25(23)31-27)33-14-12-32(2)13-15-33/h4-11,16-17H,3,12-15H2,1-2H3,(H,28,30)(H,29,31)Key: PRDFBSVERLRRMY-UHFFFAOYAR
SMILES
  • CCOc1ccc(cc1)c2c3cc(ccc3n2)c4c5cc(ccc5n4)N6CCN(CC6)C
Properties
Chemical formula C27H28N6O
Molar mass 452.562 g·mol
Except where otherwise noted, data are given for materials in their standard state (at 25 °C , 100 kPa). Infobox references
Chemical compound

Bisbenzimide (Hoechst 33342) is an organic compound used as a fluorescent stain for DNA in molecular biology applications. Several related chemical compounds are used for similar purposes and are collectively called Hoechst stains.

Application

Bisbenzimide tends to bind to adenine–thymine-rich regions of DNA and can decrease its density. Bisbenzimide mixed with DNA samples can then be used to separate DNA according to their AT percentage using a cesium chloride (CsCl) gradient centrifugation.

Nuclei of Platynereis dumerilii larvae stained with Hoechst 33342

References

  1. "bisBenzimide H 33342 trihydrochloride". Sigma-Aldrich.

External links

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