Mixture of methyl blue and lactophenol
Fusarium oxysporum conidiophores stained by lactophenol cotton blue
Lactophenol cotton blue (LCB) is a mixture of methyl blue , a histological stain , and lactophenol (a solution of phenol , lactic acid , and glycerol in water). It is used in wet-mount preparations for visualization of fungal structures, especially in medical mycology .
Methyl blue stains fungal cell walls a bright cerulean color, while lactophenol acts as a mountant .
References
J. Heritage; E. G. V. Evans; R. A. Killington (1996). Introductory Microbiology . Cambridge University Press. p. 100 . ISBN 978-0-521-44977-9 .
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