Leyser is a surname. Notable people with the surname include:
- Polykarp Leyser (disambiguation), multiple people
- Ottoline Leyser (born 1965), British plant biologist
- Henrietta Leyser (born 1941), English historian
- Karl Leyser (1920–1992), German-born British historian, husband of Henrietta and father of Ottoline
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