o-aminophenol oxidase - Misplaced Pages

o-Aminophenol oxidase (EC 1.10.3.4, isophenoxazine synthase, o-aminophenol:O₂ oxidoreductase, 2-aminophenol:O₂ oxidoreductase, GriF) is an enzyme with systematic name 2-aminophenol:oxygen oxidoreductase. This enzyme catalyses the following chemical reaction

2 o-aminophenol + O₂ + acceptor ⇌ 2-aminophenoxazin-3-one + reduced acceptor + 2 H₂O (overall reaction)

(1a) 2 2-aminophenol + O₂ ⇌ 2 6-iminocyclohexa-2,4-dienone + 2 H₂O

(1b) 2 6-iminocyclohexa-2,4-dienone + acceptor ⇌ 2-aminophenoxazin-3-one + reduced acceptor (spontaneous)

o-Aminophenol oxidase is a flavoprotein.

References

Nair PM, Vaidyanathan CS (March 1964). "Isophenoxazine synthase". Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects. 81 (3): 507–16. doi:10.1016/0926-6569(64)90135-x. PMID 14170322.
Nair PM, Vining LC (February 1965). "Isophenoxazine synthase apoenzyme from Pycnoporus coccineus". Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation. 96 (2): 318–27. doi:10.1016/0926-6593(65)90015-9. PMID 14298835.
Subba Rao PV, Vaidyanathan CS (February 1967). "Studies on the metabolism of o-aminophenol. Purification and properties of isophenoxazine synthase from Bauhenia monandra". Archives of Biochemistry and Biophysics. 118 (2): 388–94. doi:10.1016/0003-9861(67)90365-7. PMID 4166439.
Suzuki H, Furusho Y, Higashi T, Ohnishi Y, Horinouchi S (January 2006). "A novel o-aminophenol oxidase responsible for formation of the phenoxazinone chromophore of grixazone". The Journal of Biological Chemistry. 281 (2): 824–33. doi:10.1074/jbc.M505806200. PMID 16282322.

External links

O-aminophenol+oxidase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

v t e Oxidoreductases: diphenol family (EC 1.10)
1.10.1	Trans-acenaphthene-1,2-diol dehydrogenase
1.10.2	Coenzyme Q - cytochrome c reductase
1.10.3	Catechol oxidase Laccase
1.10.99	Cytochrome b6f complex
Other	Alternative oxidase

v t e Enzymes
Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Diffusion-limited enzyme Cofactor Enzyme catalysis
Regulation	Allosteric regulation Cooperativity Enzyme inhibitor Enzyme activator
Classification	EC number Enzyme superfamily Enzyme family List of enzymes
Kinetics	Enzyme kinetics Eadie–Hofstee diagram Hanes–Woolf plot Lineweaver–Burk plot Michaelis–Menten kinetics
Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list) EC7 Translocases (list)

Portal:

Biology

Category:

EC 1.10.3