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Pyrrolysine—tRNAPyl ligase

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Enzyme catalyst
Pyrrolysine—tRNA ligase
Identifiers
EC no.6.1.1.26
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Pyrrolysine—tRNA ligase (EC 6.1.1.26, PylS, pyrrolysyl-tRNA synthetase) is an enzyme with systematic name L-pyrrolysine:tRNAPyl ligase (AMP-forming). This enzyme catalyses the following chemical reaction

ATP + L-pyrrolysine + tRNAPyl {\displaystyle \rightleftharpoons } AMP + diphosphate + L-pyrrolysyl-tRNAPyl

This enzyme is specific for pyrrolysine as substrate as it cannot be replaced by lysine or any of the other natural amino acids.

References

  1. Blight SK, Larue RC, Mahapatra A, Longstaff DG, Chang E, Zhao G, Kang PT, Green-Church KB, Chan MK, Krzycki JA (September 2004). "Direct charging of tRNA(CUA) with pyrrolysine in vitro and in vivo". Nature. 431 (7006): 333–5. doi:10.1038/nature02895. PMID 15329732. S2CID 4395541.
  2. Polycarpo C, Ambrogelly A, Bérubé A, Winbush SM, McCloskey JA, Crain PF, Wood JL, Söll D (August 2004). "An aminoacyl-tRNA synthetase that specifically activates pyrrolysine". Proceedings of the National Academy of Sciences of the United States of America. 101 (34): 12450–4. doi:10.1073/pnas.0405362101. PMC 515082. PMID 15314242.
  3. Schimmel P, Beebe K (September 2004). "Molecular biology: genetic code seizes pyrrolysine". Nature. 431 (7006): 257–8. doi:10.1038/431257a. PMID 15372017.

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Enzymes: CO CS and CN ligases (EC 6.1-6.3)
6.1: Carbon-Oxygen
6.2: Carbon-Sulfur
6.3: Carbon-Nitrogen
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