Rhamnogalacturonan hydrolase

Rhamnogalacturonan hydrolase (EC 3.2.1.171, rhamnogalacturonase A, RGase A, RG-hydrolase) is an enzyme with systematic name rhamnogalacturonan alpha-D-GalA-(1->2)-alpha-L-Rha hydrolase. This enzyme catalyses the following chemical reaction

Endohydrolysis of alpha-D-GalA-(1->2)-alpha-L-Rha glycosidic bond in the rhamnogalacturonan I backbone with initial inversion of anomeric configuration releasing oligosaccharides with beta-D-GalA at the reducing end.

The enzyme is part of the degradation system for rhamnogalacturonan I in Aspergillus aculeatus.

References

Petersen TN, Kauppinen S, Larsen S (April 1997). "The crystal structure of rhamnogalacturonase A from Aspergillus aculeatus: a right-handed parallel beta helix". Structure. 5 (4): 533–44. doi:10.1016/s0969-2126(97)00209-8. PMID 9115442.
Kofod LV, Kauppinen S, Christgau S, Andersen LN, Heldt-Hansen HP, Dörreich K, Dalbøge H (November 1994). "Cloning and characterization of two structurally and functionally divergent rhamnogalacturonases from Aspergillus aculeatus". The Journal of Biological Chemistry. 269 (46): 29182–9. PMID 7961884.
Azadi P, O'Neill MA, Bergmann C, Darvill AG, Albersheim P (December 1995). "The backbone of the pectic polysaccharide rhamnogalacturonan I is cleaved by an endohydrolase and an endolyase". Glycobiology. 5 (8): 783–9. doi:10.1093/glycob/5.8.783. PMID 8720076.
Petersen TN, Christgau S, Kofod LV, Kauppinen S, Johnson AH, Larsen S (January 1997). "Crystallization and preliminary X-ray studies of rhamnogalacturonase A from Aspergillus aculeatus". Acta Crystallographica Section D. 53 (Pt 1): 105–7. doi:10.1107/s0907444996010785. PMID 15299976.
Pitson SM, Mutter M, van den Broek LA, Voragen AG, Beldman G (January 1998). "Stereochemical course of hydrolysis catalysed by alpha-L-rhamnosyl and alpha-D-galacturonosyl hydrolases from Aspergillus aculeatus". Biochemical and Biophysical Research Communications. 242 (3): 552–9. doi:10.1006/bbrc.1997.8009. PMID 9464254.

External links

Rhamnogalacturonan+hydrolase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

Hydrolase: sugar hydrolases (EC 3.2)

3.2.1: Glycoside hydrolases

Disaccharidase	Sucrase/Sucrase-isomaltase/Invertase Maltase Trehalase Lactase
Glucosidases	Cellulase Alpha-glucosidase Acid Neutral AB Neutral C Beta-glucosidase cytosolic Debranching enzyme
Other	Amylase Alpha-amylase Chitinase Lysozyme Neuraminidase NEU1 NEU2 NEU3 NEU4 Bacterial neuraminidase Viral neuraminidase Galactosidases Alpha Beta alpha-Mannosidase Glucuronidase Klotho Hyaluronidase Pullulanase Glucosylceramidase lysosomal non-lysosomal Galactosylceramidase Alpha-N-acetylgalactosaminidase NAGA Alpha-N-acetylglucosaminidase Fucosidase Hexosaminidase HEXA HEXB Iduronidase Maltase-glucoamylase Heparanase HPSE2

3.2.2: Hydrolysing
N-Glycosyl compounds

DNA glycosylases: Oxoguanine glycosylase

v t e Enzymes
Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Diffusion-limited enzyme Cofactor Enzyme catalysis
Regulation	Allosteric regulation Cooperativity Enzyme inhibitor Enzyme activator
Classification	EC number Enzyme superfamily Enzyme family List of enzymes
Kinetics	Enzyme kinetics Eadie–Hofstee diagram Hanes–Woolf plot Lineweaver–Burk plot Michaelis–Menten kinetics
Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list) EC7 Translocases (list)

Portal:

Biology

Category:

EC 3.2.1