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tRNA pseudouridine synthase (EC 5.4.99.25, TruB, aCbf5, Pus4, YNL292w (gene), Psi55 tRNA pseudouridine synthase, tRNA:Psi-synthase, tRNA pseudouridine 55 synthase, tRNA:pseudouridine-55 synthase, Psi synthase, tRNA Psi synthase, tRNA:Psi synthase, tRNA-uridine uracil mutase, Pus10, tRNA-uridine uracil mutase) is an enzyme with systematic name tRNA-uridine uracil mutase. This enzyme catalyses the following chemical reaction

tRNA uridine ${\displaystyle \rightleftharpoons }$ tRNA pseudouridine

Pseudouridine synthase TruB from Escherichia coli specifically modifies uridine in tRNA molecules.

References

1. Nurse K, Wrzesinski J, Bakin A, Lane BG, Ofengand J (March 1995). "Purification, cloning, and properties of the tRNA psi 55 synthase from Escherichia coli". RNA. 1 (1): 102–12. PMC 1369054. PMID 7489483.
2. Becker HF, Motorin Y, Planta RJ, Grosjean H (November 1997). "The yeast gene YNL292w encodes a pseudouridine synthase (Pus4) catalyzing the formation of psi55 in both mitochondrial and cytoplasmic tRNAs". Nucleic Acids Research. 25 (22): 4493–9. doi:10.1093/nar/25.22.4493. PMC 147073. PMID 9358157.
3. Pieńkowska J, Wrzesiński J, Szweykowska-Kulińska Z (1998). "A cell-free yellow lupin extract containing activities of pseudouridine 35 and 55 synthases". Acta Biochimica Polonica. 45 (3): 745–54. doi:10.18388/abp.1998_4268. PMID 9918501.
4. Chaudhuri BN, Chan S, Perry LJ, Yeates TO (June 2004). "Crystal structure of the apo forms of psi 55 tRNA pseudouridine synthase from Mycobacterium tuberculosis: a hinge at the base of the catalytic cleft". The Journal of Biological Chemistry. 279 (23): 24585–91. doi:10.1074/jbc.m401045200. PMID 15028724.
5. Hoang C, Hamilton CS, Mueller EG, Ferré-D'Amaré AR (August 2005). "Precursor complex structure of pseudouridine synthase TruB suggests coupling of active site perturbations to an RNA-sequestering peripheral protein domain". Protein Science. 14 (8): 2201–6. doi:10.1110/ps.051493605. PMC 2279332. PMID 15987897.
6. Gurha P, Gupta R (December 2008). "Archaeal Pus10 proteins can produce both pseudouridine 54 and 55 in tRNA". RNA. 14 (12): 2521–7. doi:10.1261/rna.1276508. PMC 2590954. PMID 18952823.

External links

• TRNA+pseudouridine55+synthase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

• Biology

• EC 5.4.99