In genetic engineering, transposon tagging is a process where transposons (transposable elements) are amplified inside a biological cell by a tagging technique. Transposon tagging has been used with several species to isolate genes. Even without knowing the nature of the specific genes, the process can still be used.
In plants
By molecular separation of transposons, from a cell nucleus, the cloning is enabled for genes which contain the transposons.
By using transposon tagging, researchers have been able to add genetic elements from maize (corn) and Antirrhinum into some other species (such as tobacco, aspen and others). A gene responsible for a particular phenotype can be cloned within a given species, when movement is accompanied by the presence of a mutant phenotype.
References
- ^ Shah, Paaras V. (2007). "Transposable Elements".
- ^ McClean, Phillip (1998). "Transposon Tagging".
- Brutnell, Thomas (2002). "Transposon tagging in maize". Functional & Integrative Genomics. 2 (1–2): 4–12. doi:10.1007/s10142-001-0044-0. PMID 12021846. S2CID 23266030.
- Dinesh-Kumar, S. P.; Whitham, S.; Choi, D.; Hehl, R.; Corr, C.; Baker, B. (1995). "Transposon Tagging of Tobacco Mosaic Virus Resistance Gene N: Its Possible Role in the TMV-N-Mediated Signal Transduction Pathway". Proceedings of the National Academy of Sciences of the United States of America. 92 (10): 4175–4180. Bibcode:1995PNAS...92.4175D. doi:10.1073/pnas.92.10.4175. JSTOR 2367289. PMC 41906. PMID 7753780.
- Fladung, M; Deutsch, F; Hönicka, H; Kumar, S (2004). "T-DNA and Transposon Tagging in Aspen". Plant Biology. 6 (1): 5–11. doi:10.1055/s-2003-44745. PMID 15095129. S2CID 260252516.
Further reading
- Transposon Tagging, North Dakota State University
- A.M. Settles, Transposon Tagging and Reverse Genetics, University of Florida
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