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In enzymology, a tubulin—tyrosine ligase (EC 6.3.2.25) is an enzyme that catalyzes the chemical reaction

ATP + detyrosinated α-tubulin + L-tyrosine ${\displaystyle \rightleftharpoons }$ α-tubulin + ADP + phosphate

The 3 substrates of this enzyme are ATP, detyrosinated alpha-tubulin, and L-tyrosine, whereas its 3 products are alpha-tubulin, ADP, and phosphate.

This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds as acid-D-amino-acid ligases (peptide synthases). The systematic name of this enzyme class is alpha-tubulin:L-tyrosine ligase (ADP-forming).

References

• Wehland J, Schroder HC, Weber K (1986). Isolation and purification of tubulin tyrosine ligase. Methods Enzymol. Vol. 134. pp. 170–9. doi:10.1016/0076-6879(86)34086-2. ISBN 978-0-12-182034-3. PMID 3821560.
• Rudiger M, Wehland J, Weber K (1994). "The carboxy-terminal peptide of detyrosinated alpha tubulin provides a minimal system to study the substrate specificity of tubulin-tyrosine ligase". Eur. J. Biochem. 220 (2): 309–20. doi:10.1111/j.1432-1033.1994.tb18627.x. PMID 7510228.

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