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BAF agar or biotin-aneurin-folic acid agar is a type of agar growth medium containing peptones. It is used to grow cultures of mycorrhizal fungi. It was first described by A.J.P. Oort [Wikidata] in Nutritional requirements of Lactarius species and cultural characters in relation to taxonomy in 1981. The acidic pH (5.8-6.3) of BAF agar inhibits bacterial growth.

Typical composition

BAF agar typically contains:

• 30.0 g/L glucose
• 2.0 g/L peptone
• 0.2 g/L yeast extract
• 0.5 g/L KH₂PO₄
• 0.5 g/L MgSO₄.7 H₂O
• 10.0 mg/L FeCl₃.6 H₂O
• 1.0 mg/L ZnSO₄.7 H₂O
• 5.0 mg/L MnSO₄
• 100.0 mg/L CaCl₂.2 H₂O
• 50.0 μg/L thiamine HCl
• 1.0 μg/L biotin
• 100.0 μg/L folic acid
• 50.0 μg/L inositol
• 15 g/L agar

References

1. ^ Águeda, Beatriz; Parladé, Javier; Fernández-Toirán, Luz Marina; Cisneros, Óscar; de Miguel, Ana María; Modrego, María Pilar; Martínez-Peña, Fernando; Pera, Joan (October 2008). "Mycorrhizal synthesis between Boletus edulis species complex and rockroses (Cistus sp.)". Mycorrhiza. 18 (8): 443–449. Bibcode:2008Mycor..18..443A. doi:10.1007/s00572-008-0192-3. ISSN 0940-6360. PMID 18695982. S2CID 11233267.
2. ^ "CBS List of Media" (PDF).

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