Misplaced Pages

diadenylate cyclase
Diadenylate cyclase homooctamer, Thermotoga maritima
Identifiers
EC no.	2.7.7.85
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Diadenylate cyclase EC 2.7.7.85, DNA integrity scanning protein DisA is a DNA binding protein participates in a DNA-damage check-point. DisA forms globular foci that rapidly scan along the chromosomes searching for lesions. Catalytic activity

2 ATP ${\displaystyle \rightleftharpoons }$ 2 diphosphate + cyclic di-3',5'-adenylate.

This enzyme has diadenylate cyclase activity, catalyzing the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP). c-di-AMP likely acts as a signaling molecule that may couple DNA integrity with a cellular process. This rate-limiting step is the accessibility of the active site; mutating the possible exit tunnel (residues 128-130) increases product 2-fold despite Arg-130 being important for ATP-binding. Does not convert GTP to c-di-GMP.

References

1. Witte G, Hartung S, Büttner K, Hopfner KP (April 2008). "Structural biochemistry of a bacterial checkpoint protein reveals diadenylate cyclase activity regulated by DNA recombination intermediates". Molecular Cell. 30 (2): 167–78. doi:10.1016/j.molcel.2008.02.020. PMID 18439896.

• Biology

• EC 2.7.7
• Bacterial proteins