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Mathurapur Union (Bengali: মথুরাপুর ইউনিয়ন) is a union parishad situated at Daulatpur Upazila, in Kushtia District, Khulna Division of Bangladesh. The union has an area of 69.93 square kilometres (27.00 sq mi) and as of 2001 had a population of 33,823. There are 11 villages and 8 mouzas in the union.