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Route 94 commenced operating on 22 September 1990 to replace the withdrawn section of route 88 between Acton Green and Oxford Circus, it then ran parallel to route 88 to Trafalgar Square (Sundays only). On 13 July 1991 it was diverted to East Acton DuCane Road and the service level increased following the truncation of route 12 west of Shepherd's Bush. At the same time it was extended at all times to Trafalgar Square.
Route 94 gained a night service in the form of N94 in July 1999. It was the first night bus and was introduced by the General.
Route 94 was featured on the BBC Radio 4 program Today in December 2003 as part of a story by guest editor Gillian Reynolds on the withdrawal of the Routemaster. It also appeared on the front of the annual Britain's Buses calendar for the same year.